á-PSP May Prevent Neurovulnerability in Alzheimer Model in Vitro

Objective: The current study investigated the neurotrophic and neuroprotective action of a unique formulation of á-PSP which consists of carbohydrate, crude protein, and essential minerals by using pressure and mechanical hydrolysis to make a complex formulation designated alpha polysaccharidepeptides (á-PSP). Methods: Using neuronal cell lines prepared from the LA-N-5 (Los Angeles Neuroblastoma) for Alzheimer's disease (AD) in complete media and treated with indicated manner, inverted microscopic evaluated morphological and biochemical analysis were conducted to determine the neurotrophic and neuroprotective properties of á-PSP. Results: Results of these analysis demonstrated that á-PSP significantly decreased neuronal cell death, a cellular marker of memory formation. Dose response analysis (experiment going on) indicated that the lowest effective concentration of á-PSP exerted the minimal neurotrophic effect. Results of neuroprotection studies demonstrated that á-PSP induced highly significant neuroprotection against beta-amyloid, hydrogen peroxide, and glutamate-induced toxicity. Discussion: Abnormality of glucose/energy metabolism shows relation to AD (1, 2, 3, 4). á-PSP may prevent impairment of glucose/energy metabolism and may improve the ability of neurons to reduce the levels of (" scavenger ") free radicals and thereby affecting ATP levels (11, 12). Conclusion: á-PSP induced cellular markers of memory function in neurons critical to memory and vulnerable to negative effects of aging, cellular degeneration and Alzheimer's disease. Results of the current study could demonstrate the cellular mechanism of á-PSP on cognitive function and a possible intervention in Alzheimer's disease. Abstract (Preliminary data) 2 1.1 Alzheimer's disease: A scientific mystery and major impact. Abnormality of glucose/energy metabolism shows relation to Alzheimer's disease (1, 2, 3, 4). Degenerative and cell death are major causes in AD. 1.2 á-PSP is a complex formulation that consists of carbohydrate, crude protein and essential minerals by using pressure and mechanical hydrolysis to make a complex formulation designated alpha polysaccharidepeptides (á-PSP) (5). 1.3 á-PSP is very safe because it contains phytochemicals that have components of carbohydrates, crude protein and essential minerals by using pressure and mechanical hydrolysis to make a complex formulation designated á-PSP. Evidence by observation from animal (pig) data showed that á-PSP can decrease morbidity from Ataxia (á-PSP may improve cerebral blood flow). In clinical use we found that á-PSP improves short and long term memory (6).